Coming soon.
We will share the protocols, methods, algorithms and etc for single-molecule and super-resolution imaging methods.
The research interests of the cell biology field are now shifting from cellular to molecular level, from qualitative to quantitative characterization and from micro- to nano-meter scale. This process is benefited from the rapid development of new biophysical nanotools that offer high spatial and temporal resolutions. The biophysical nanotools allow the biologists to observe the dynamics and interactions of single molecules, organelles, cells to organoids with ultra-sensitivity and low cell toxicity. Here, in this resources titled “Biophysical nanotools for single-molecule dynamics”, we would like to share the biophysical techniques into two major classes: i) the mechanical nanotools like dynamic force spectroscopy (DFS) and Biomembrane Fore Probes (BFP) and ii) the optical nanotools like single-molecule and super-resolution microscopy. We will also discuss the applications in elucidating the cellular and molecular dynamics and functionally mapping of interactions between inter-cellular networks and intra-cellular components, which is the key to understanding cellular processes such as adhesion, trafficking, inheritance, and division.
We will share the protocols, methods, algorithms and etc for single-molecule and super-resolution imaging methods.
The research interests of the cell biology field are now shifting from cellular to molecular level, from qualitative to quantitative characterization and from micro- to nano-meter scale. This process is benefited from the rapid development of new biophysical nanotools that offer high spatial and temporal resolutions. The biophysical nanotools allow the biologists to observe the dynamics and interactions of single molecules, organelles, cells to organoids with ultra-sensitivity and low cell toxicity. Here, in this resources titled “Biophysical nanotools for single-molecule dynamics”, we would like to share the biophysical techniques into two major classes: i) the mechanical nanotools like dynamic force spectroscopy (DFS) and Biomembrane Fore Probes (BFP) and ii) the optical nanotools like single-molecule and super-resolution microscopy. We will also discuss the applications in elucidating the cellular and molecular dynamics and functionally mapping of interactions between inter-cellular networks and intra-cellular components, which is the key to understanding cellular processes such as adhesion, trafficking, inheritance, and division.