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Surface Cleaning

[Protocol - Washing Labtek Chambers for Single-Molecule Imaging]
for 8-well LabTek dishes
​
Remove the lids, arrange the Labtek dishes in a beaker, make sure the coverglass is vertical (as ultrasound is standing wave).
Add 1M KOH (Sigma-Aldrich) carefully into the beaker and make sure all the small wells are full of liquid (IMPORTANT!!!).
Sonicate the dishes for 30min to remove "residue" and add -OH to the SiO2.
Rinsed with large amounts of ddH2O (MilliQ) 4~5 times;
Sonicate coverslip in ddH2O (MilliQ) again for 10min to remove the KOH.
Briefly remove the water in the wells and air-dry overnight, under the shelf to avoid dust.
Check the autofluo dots on the surface with TIRF. (should be less than 20 dots per field of view)
Ready to use.
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Site edited by Dr Qian Peter Su
Last update: 14 Jan 2023
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  • Home
  • Our research
    • Autophagy and Mitochondria
    • Chromatin DNA Structure
    • Cardiovascular Interactome
    • Single-Mol and Super-Res Tech
  • Publications
  • Our Team
    • Team Members
    • About Peter Su
    • Team Photos
    • Our Collaborators
  • Lab News
  • Resources
    • Facility and Infrastructure
    • Surface Cleaning
    • Antibody Labelling
    • Chromatin DNA Labelling
    • Immunostaining
    • Actin Fluorescent Labelling
    • Motor Protein Purification
    • in vitro Reconstitution
    • Single-molecule Tracking
    • Super-resolution Mapping
    • Codes for SM and SR
  • Contact us
  • Mix Bag
  • Intranet